A novel protein increases heat stress tolerance in plants
Enhancement of gene expression without growth retardation
Graduate School of Agricultural and Life Sciences / Faculty of Agriculture
Researchers at the University of Tokyo, Japan International Center for Agricultural Sciences and RIKEN have demonstrated a novel molecular mechanism that regulates gene expression in response to heat stress in plants.
Plants respond to environmental stresses such as drought, high-salinity and heat, and induce expression of numerous stress tolerance genes in order to adapt to the stress conditions. Arabidopsis DREB2A is an important transcription factor that regulates expression levels of many heat stress-responsive genes that function in acquisition of stress tolerance under heat stress conditions. However, the detailed mechanism of how the DREB2A protein activates the target genes remain to be elucidated.
Graduate School of Agricultural and Life Sciences Professor Kazuko Yamaguchi-Shinozaki and co-workers identified DPB3-1 as a novel interacting protein with DREB2A in Arabidopsis thaliana and revealed that the DPB3-1 bound to DREB2A and was involved in transcriptional regulation of DREB2A. Overexpression of the DPB3-1 enhanced the expression levels of many heat stress-inducible genes including the DREB2A target genes that were important to increase heat stress tolerance for plants under heat stress conditions. Moreover, the DPB3-1-overexpressing plants showed enhanced heat stress tolerance and did not show any growth retardation under non-stress conditions.
Heat stress is expected to be a major problem affecting crop productivity as a result of global warming. The current research suggests a new mechanism by which plants acquire heat-stress resistance, the application of which can be used for plant breeding to generate crops with enhanced heat stress tolerance.
Hikaru Sato, Junya Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, Teppei Ohori, Kazuya Kusakabe, Maika Nagata, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki,
“Arabidopsis DPB3-1, a novel DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits”,
The Plant Cell Online Edition: 2014/12/9 (Japan time), doi: 10.1105/tpc.114.132928.