Cell sheet technology combined with TD-198946
Towards a treatment for osteoarthritis
Graduate School of Medicine / Faculty of Medicine
Graduate School of Engineering / Faculty of Engineering
Articular cartilage is a permanent tissue with poor self-regenerative capacity. Consequently, a tissue engineering approach to cartilage regenerative therapy could greatly advance the current treatment options for patients with cartilage degeneration and/or defects. Project Research Associate Fumiko Yano and Prof. Ung-il Chung and their colleagues at the Graduate School of Engineering and Graduate School of Medicine, previously reported that direct injection of the thienoindazole derivative, TD-198946, into the knee joints of mice halted the progression of osteoarthritis; the compound induced chondrogenic differentiation without promoting endochondral ossification. In the present study, the researchers applied TD-198946 to a cell-based cartilage reconstruction model, taking advantage of cell-sheet technology (Cell Seed Inc.). The transplanted cell-sheets with TD-198946 were then successfully used to promote the reconstruction of permanent cartilage, with no evidence of chondrocyte hypertrophy in the knee articular cartilage defects created in mice and canines.
This study has demonstrated cell-sheet technology combined with TD-198946, a non-conventional technique that realizes articular cartilage reconstruction without scaffolds or cytokines, thus achieving a significant step forward in the treatment of osteoarthritis.
Fumiko Yano, Hironori Hojo, Shinsuke Ohba, Taku Saito, Muneki Honnami, Manabu Mochizuki, Tsuyoshi Takato, Hiroshi Kawaguchi, Ung-il Chung,
“Cell-sheet technology combined with a thienoindazole derivative small compound TD-198946 for cartilage regeneration”,
Biomaterials Online Edition: 2013/4/24, doi: 10.1016/j.biomaterials.2013.04.008.