Construction of “Quenchbodies” Quench-Based Antibody Probes showing Antigen-Dependent Fluorescence
While antibodies have been utilized in detecting a range of molecules (antigens) in various settings from basic biological research to clinical diagnostics, there has been no easy and versatile way to detect their binding to their cognate antigens in a liquid sample.
?Associate Professor Hiroshi Ueda in the University of Tokyo’s School of Engineering and co-researchers at JAIST and ProteinExpress Corporation used a position-specific protein-labeling technology to develop a novel method for making fluorescence-labeled antibody fragments (Quenchbodies) whose fluorescence is quenched by the internal amino acids in the antibody alone, but is markedly increased upon binding to the antigen. As a result, the method enables easy and rapid detection of various molecules including biomarker peptides and proteins as well as small chemicals such as bisphenol A, morphine and heroin in part per billion concentrations, by simple “mix and match” measurements. The method is expected to lead to the development of innovative detection and diagnostic tools and devices.
R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka, and H. Ueda,
“”Quenchbodies” : quench-based antibody probes that show antigen-dependent fluorescence”,
Journal of the American Chemical Society Online Edition: 2011 / 10 / 7（Japan time）